biotinylated wheat germ agglutinin (Vector Laboratories)

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Vector Laboratories biotinylated wheat germ agglutinin
Biotinylated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+wheat+germ+agglutinin/Biotinylated+Wheat+Germ+Agglutinin+(WGA)/us12605429-1021-16-20
Average 94 stars, based on 268 article reviews

biotinylated wheat germ agglutinin - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility
Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [anti-PNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility.
Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [antiPNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

Article Title: Proteins having pneumococcal capsule degrading activity and methods of use
Article Snippet: .. For microscopy, cells were incubated at room temperature for 30 minutes with a 1/500 dilution of biotinylated wheat germ agglutinin (Vector labs) in 1% bovine serum albumin followed by a 30-minute room temperature incubation with a 1/1000 dilution of streptavidin APC (Biolegend). ..

Article Title: Fusions with CD8 antigen binding molecules for modulating immune cell function
Article Snippet: .. 25 μL of 5 μg/mL biotinylated wheat germ agglutinin (Vector Labs), which binds to glycoconjugates, in PBS was added and incubated at room temperature for 30 mins. ..

Whole Genome Amplification:

Article Title: Butyrate Rescues Cardiac Metabolic Dysfunction in Hypertensive Heart Failure with Preserved Ejection Fraction
Article Snippet: .. Primary antibody (Rabbit anti-rat Fibronectin antibody [1:75] Ab23751; Abcam, Mouse anti-rat CD68 antibody | Clone ED1 [1:50] MCA341R; Bio-Rad) or biotinylated wheat germ agglutinin (WGA [1:100] B1025 VectorLab) or sirius red solution (0.1% in picric acid, Sigma) was diluted in 10% NDS and applied to the slice. .. After incubation in a humidity chamber overnight (4°C) or 60 min (Sirius red), excess antibody was removed, Cy3-conjugated secondary antibody (715-165-151, 711-165-152, respectively; Jackson ImmunoResearch) was diluted 1:100 or 1:300 in 1x PBS and incubated in a humidity chamber (120 min at RT).

Article Title: The acetylglucosaminyltransferase GnT-Ⅲ regulates erythroid differentiation through ERK/MAPK signaling.
Article Snippet: .. The experiments were performed using the following antibodies and reagents: Antibodies against CD71 (13113), c-Kit (3074), p44/42 MAPK (ERK1/2; 9102), Phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK1/2; 4370), SAPK/JNK (9252), Phospho-SAPK/JNK (Thr183/Tyr185) (4668), p38 MAPK (9212), Phospho-p38 MAPK (Thr180/Tyr182) (4511), the peroxidase-conjugated secondary antibody against rabbit (7074S) and U0126 (9903) were purchased from Cell Signaling Technology; Biotinylated Phaseolus vulgaris erythroagglutinin (E4-PHA; B-1385), Datura stramonium agglutinin (DSA; B-1185), biotinylated Phaseolus Vulgaris Leucoagglutinin (L4-PHA; B-1115–2), Jo urn al Pr e-p roo f 12 biotinylated S. nigra agglutinin (SNA; B1305), biotinylated Wheat germ agglutinin (WGA; B-1025-5) and ABC kit (PK-4000) were from Vector Laboratories; Biotinylated Lens culinaris agglutinin (LCA; J207) and E4-PHA agarose (J311) were obtained from J-Oil Mills, Japan; Biotinylated M. amurensis (MAM) lectin (#BA-s7801-2) was purchased from EY Laboratories; The anti-α-Tubulin (T6199) antibody and peroxidase-conjugated secondary agents against mouse (AP124P), Sodium butyrate (NaBu) (303410), SAHA (HY-10221), hemin (HY-19424) were acquired from Sigma; The antibody against CD235a (JC159) was from Thermo Fisher; The goat anti-mouse IgG Alexa Fluor 568, goat anti-rabbit IgG Alexa Fluor 488 and streptavidin conjugate Alexa Fluor 647 antibody were from Invitrogen; Phosphotyrosine (p-Tyr) antibody (sc-7020; USA) was from Santa Cruz; Peptide-NGlycosidase F (PNGase F) was from Roche. .. The cycloheximide (CHX, 037-20991) was from Wako, Japan; Cell Counting Kit-8 (CCK-8) was obtained from DOJINDO (341-07761; Japan).

other:

Article Title: The acetylglucosaminyltransferase GnT-Ⅲ regulates erythroid differentiation through ERK/MAPK signaling
Article Snippet: The experiments were performed using the following antibodies and reagents: Antibodies against CD71 (13113), c-Kit (3074), p44/42 MAPK (ERK1/2; 9102), phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK1/2; 4370), SAPK/JNK (9252), phospho-SAPK/JNK (Thr183/Tyr185) (4668), p38 MAPK (9212), phospho-p38 MAPK (Thr180/Tyr182) (4511), the peroxidase-conjugated secondary antibody against rabbit (7074S), and U0126 (9903) were purchased from Cell Signaling Technology; biotinylated E4-PHA (B-1385), DSA (B-1185), biotinylated L4-PHA (B-1115–2), biotinylated SNA (B-1305), biotinylated Wheat germ agglutinin (B-1025-5), and ABC kit (PK-4000) were from Vector Laboratories; biotinylated LCA (J207) and E4-PHA agarose (J311) were obtained from J-Oil Mills; biotinylated MAM lectin (#BA-s7801-2) was purchased from EY Laboratories; The anti-α-Tubulin (T6199) antibody and peroxidase-conjugated secondary agents against mouse (AP124P), NaBu (303410), SAHA (HY-10221), and hemin (HY-19424) were acquired from Sigma; The antibody against CD235a (JC159) was from Thermo Fisher Scientific; The goat anti-mouse IgG Alexa Fluor 568, goat anti-rabbit IgG Alexa Fluor 488 and streptavidin conjugate Alexa Fluor 647 antibody were from Invitrogen; phosphotyrosine (p-Tyr) antibody (sc-7020) was from Santa Cruz; PNGase F was from Roche.

Microscopy:

Avidin-Biotin Assay:

Article Title: Profiling of peptides from wet- and dry-heated milk proteins after in vitro infant digestion and intestinal transport, and their effects on mucus production and innate immunity.
Article Snippet: QPCR primers were purchased from Biolegio BV. .. Biotinylated wheat germ agglutinin (B-1025) and avidin-peroxidase conjugate from the VECTASTAIN® Elite® ABC-HRP Kit (PK-6100) were obtained from Vector Laboratories, and sulfuric acid was obtained from Brunschwig. .. Porcine pepsin (P6887), porcine pancreatin (P7545), porcine bile extract (B8631), Dulbecco’s Phosphate buffered saline (DPBS) with and without CaCl2 +/MgCl2+, prostaglandin E2 (PGE2), mucin from porcine stomach (M1778), bovine serum albumin (BSA; A3059), o-phenylenediamine dihydrochloride (P8787), Alcian Blue 8GX (A5268), PAS staining kit (1.1646.0001) and all other chemicals were obtained from Sigma Aldrich.

Review